Teaching experience (4 years)

Genetic Engineering, Plant and Animal Biotechnology, Plant Functional Genomics, Genomics and Proteomics, Genetic Engineering,  Biochemistry, Biophysics and Instrumentation, Cell and Molecular Biology (PG course, all the papers having 4 credits each) and Genetic Engineering (UG course, 4 credit)

1. Transgenic Tea Over-expressingSolanum tuberosum Endo-1,3-beta-D-glucanase Gene Conferred Resistance Against Blister Blight Disease (2018). H. Ranjit Singh, Pranita Hazarika, Niraj Agarwala, Neelakshi Bhattacharyya & Prasenjit Bhagawati, Bornali Gohain, Tirthankar Bandyopadhyay, Raju Bharalee, Sushmita Gupta, Manab Deka3 & Sudripta Das., Plant Molecular Biology Reporter(2018) 36:107–122 (IF 1.6)
2. Morphogenesis of edible gall in Zizania latifolia (Griseb.) Turcz. ex Stapf due to Ustilago esculenta Henn. infection in India (2016) S. K. Sinha, A. C. Deka an R. Bharalee, African Journal of Microbiology Research, Vol. 10(31), pp. 1215-1223, 21 August, 2016.
3. Production of biodiesel from freshwater microalgae and evaluation of fuel properties based on fatty acid methyl ester profile.  January 2016, Swapan Sinha, Arghyadeep Gupta, Raju Bharalee,  Biofuels 7(1):69-78
4. Molecular Landscape of Helopeltis theivoraInduced Transcriptome and Defense Gene Expression in Tea - Tirthankar Bandyopadhyay ,Bornali Gohain, Raju Bharalee, Sushmita Gupta, Priyadarshini Bhorali, Sourabh Kumar Das, Mohan C. Kalita ,Sudripta Das. Plant Mol Biol Rep, DOI 10.1007/s11105-014-0811-4 (IF 1.6)
5. Identification,  cloning  and  heterologous  expressionof  a  new  lectin  like  protein  in  tea- Tirthankar  Bandyopadhyay,  Bornali  Gohain,  Raju  Bharalee, Prasenjit  Bhagawati, Mohan  Chandra  Kalita,  Sudripta  Das. J. of applied Res on Medicinal and Aromatic Plants, Vol: 1(2)43-49, 2014. (IF1.966)
6. Bioinformatics tools for development of fast and cost effective simple sequence repeat (SSR), and single nucleotide polymorphisms (SNP) markers from expressed sequence tags (ESTs)- Sushmita Gupta, Raju Bharalee, Ranjita Das, Debajit Thakur. African Journal of Biotechnology,  Vol: 12(30):4713-4721 (2013)
7. Molecular cloning, expression and computational analysis of a water stress inducible copper-containing amine oxidase gene (CuAO) from tea plant [Camellia sinensis (L.) O. Kuntze]. - R. Bharalee, S. Gupta, T. Bandyopadhyay, B. Gohain, N. Agarwala, M. C. Kalita,  and S. Das.  African Journal of Biotechnology, Vol. 11(89): 15547-15555 (2012). (IF 0.5)
8. Identification of drought tolerant progenies in tea by gene expression analysis – S. Gupta, R. Bharalee, P. Bhorali, T. Bandyopadhyay, B Gohain, N Agarwal, P Ahmed, H. Saikia, S. Borchetia, M. C. Kalita, A. K. Handique and S. Das. Funct  Integr Genomics, 12(3): 543-63 (2012)(IF 2.74)
9. Molecular analysis of drought tolerance in tea by cDNA AFLP based transcript profiling – S. Gupta, R. Bharalee, P. Bhorali, S. K. Das, P. Bhagawati, T. Bandyopadhyay, B. Gohain, N. Agarwal, P. Ahmed, S. Borchetia, M. C. Kalita, A. K. Handique and S. Das. Mol Biotechnol, 2012(IF 1.71)
10. Isolation of functional RNA from heavily infested, wilted, and necrotic leaf tissues of tea with high polyphenol content – T. Bandyopadhyay, R. Bharalee, B. Gohain, S. Gupta, N. Agarwal, H. R. Singh, Setu Chakraborty, P. Bhorali, Mohan C. Kalita and S. Das. J Agrcl Sci Technol, B2 (2012) 121-127. (IF 0.43)
11. Molecular analysis and expression profiling of blister blight defense-related genes in tea- P. Bhorali,  B. Gohain,  S. Gupta,  R. Bharalee,  T.  Bandyopadhyay,  S. K. Das,  N. Agarwal, H. R. Singh, P. Bhagawati, N. Bhattacharyya, P. Ahmed, S. Borchetia, S. Sarm,  and S. Das. Indian Journal of genetics and Plant Breeding (The), 72(2): 226-233 (2012).
12. Rubisco-bis-phosphate oxygenase (RuBP)- A potential housekeeping gene for qPCR assays in tea – B. Gohain, T. Bandyopadhyay, P. Bhorali, S. Borchetia, R. Bharalee, S. Gupta, N. Agarwala,  N. Bhattacharyya, R. Singh, P. Bhagawati, P. Ahmed, M. C. Kalita, and S. Das. African Journal of Biotechnology, Vol. 11(51), pp. 11193-11199, 26 June, 2012 (IF 0.5)
13. Heterologous expression and in-silico characterization of pathogenesis related protein1 (CsPR1) gene from Camellia sinensis – N. Agarwala, P. Bhorali, N. Bhattacharyya, R. Bharalee, T. Bandyopadhyay, B. Gohain, S. Gupta, M. Deka, S. Das. J Biochem Tech (2014) 5(2):674-678
14. Cloning and heterologous expression of a gene encoding lycopene-epsilon-cyclase, a precursor of lutein in tea (Camellia sinensis var assamica) – S. Borchetia, C. Bora, B. Gohain, P. Bhagawati, N. Agarwal, N. Bhattacharrya, R. Bharalee, P. Bhorali, T. Bandyopadhyay, S. Gupta, S. K. Das, H. R. Singh, P. Ahmed, M. Gogoi and  S. Das. African Journal of Biotechnology, 2011, 10(32): 5934-5939. (IF 0.5)
15. In vitro clonal propagation of Curcuma caesia  Rxb. and Curcuma zedoaria Rosc from rhizome  bud explants – R. Bharalee, A. Das, M C Kalita, J Plant Bioch Biotech, 2005. 14:61 – 63 (IF 1.038)
16. 2300 ESTs submitted to dbEST - (Gene Bank Acc. no.  HS393200 to HS395561).
17. Six full length gene submitted to NCBI (Acession no. JN561073- JN561077 and JN391508)

1. Book Title: Gene Networks Involved in Water Deficit stress Response in Tea: Towards genetic engineering and molecular breeding for drought stress tolerance. (LAP Lambert Academing Publishing, Germany, ISBN No. 978-3-659-29025-1)

2. Book Title: Deciphering Transcriptome Derived Drought Tolerance Mechanism in Tea-Exploring Candidate Genes for Enhance Drought Tolerance. Lambert Academic Publishing, Germany,  ISBN No. 978-620-2-52329-5

Abstract Presented in Conferences/seminar

1. R. Bharalee, S. Gupta, T. Bandyopadhyay, B. Gohain, P. Bhorali, S. K. Das, N. Agarwal, P. Ahmed, P. Bhagawati, N. Bhatacharrya, M.C. Kalita, S. Das (2011). Comparative analysis of drought responsive transcriptome in Tea (Camellia sinensis) genotypes with contrasting drought tolerance. ‘International Symposium and Workshop on Functional Genomics and Structural Biology’ (May, 09-13, 2011), University Putra Malaysia, Kuala Lampur, Malaysia.
2. R. Bharalee, T. Bandyopadhyay, S. Das. (2009). Transcriptome and EST analysis in Drought   Induced Tea – National seminar organized by Gauhati University, India, Feb, 2009.
3. R. Bharalee, A. Das, M. C. Kalita. (2005). In Vitro Clonal Propagation of Curcuma caesia Roxb. and Curcuma zedoaria Rosc. From Rhizome Bud Explants- National seminar organized by Gauhati University, India, 2005.
4. P. Bhorali, B. Gohain, S. Gupta, R. Bharalee, T. Bandyopadhyay, S. K. Das, N. Agarwal, H. R. Singh, P. Bhagawati, N. Bhattacharyya, P. Ahmed, S. Borchetia, S. Sarma and S. Das. (2012). Molecular Analysis and Expression Profiling of Blister Blight Defense-Related Genes in Tea. ‘National Seminar on Plant Genetic Research for Eastern and North-eastern India’ (May, 11-12, 2012), ICAR Research Complex for NEH Region, Meghalaya, India.
5. S. Gupta, R. Bharalee, P. Bhorali, B. Gohain, T. Bandyopadhyay, S. K. Das, P. Ahmed, P. Bhagawati, N. Bhattacharya, R. Singh, N. Agarwal, S. Borchetia, A.K. Handique and S. Das (2010). ‘Functionally relevant microsatellites in Biotic and Abiotic stress induced Unigenes of Tea’. 18^th International Conference (Post ISCBS-2012) Perspective and Challenges in Chemical and Biological Sciences: Innovation Cross Roads. IASST, 28^th – 30^th January, 2012.
6. T. Bandyopadhyay, B. Gohain, R. Bharalee, P. Bhorali, S. Gupta, N. Agarwal, H. R. Singh, P. Bhagawati, N. Bhattacharya, P. Ahmed and S. Das (2011). Stress transcriptome and tea. ‘World Tea Science Congress’ (Nov, 22-24, 2011), Tocklai Experimental Station, TRA, Jorhat, Assam, India.
7. P. Bhorali, B. Gohain, S. Gupta, R. Bharalee, T. Bandyopadhyay, S. Das, N. Agarwal, R. Singh, P. Bhagawati, P. Ahmed, N. Bhattacharyya, S. Borchetia, S. Sarma, and S. Das (2010). Defence Oriented Changes in the Tea Transcriptome upon Infection by Exobasidium Vexans. ‘12^th International PhD Student Symposium’ (Oct, 21-23, 2010), European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
8. P. Bhorali, B. Gohain, S. Gupta, R. Bharalee, T. Bandyopadhyay, S. Das, N. Agarwal, R. Singh, P. Bhagawati, P. Ahmed, N. Bhattacharyya, S. Borchetia, S. Sarma, and S. Das (2010). Screening for molecular markers associated with blister blight tolerance in tea through cDNA-AFLP based transcript profiling. ‘International Conference on Frontiers in Biological Sciences’ (Oct, 01-03, 2010), NIT, Rourkela, India.
9. S. Gupta, R. Bharalee, P. Bhorali, B. Gohain, T. Bandyopadhyay, S. K. Das, P. Ahmed, P. Bhagawati, N. Bhattacharya, R. Singh, N. Agarwal, S. Borchetia, A.K. Handique and S. Das (2010). Molecular breeding and functional genomics for abiotic stress tolerance in tea. ‘Asian Regional Conference on Systems Biology’ (Nov, 29- Dec, 01, 2010), Kuala Lumpur, Malaysia.
10. T. Bandyopadhyay, R. Bharalee, B. Gohain, S. Gupta, P. Bhorali, S. K. Das, N. Agarwal, H. R. Singh, P. Ahmed, P. Bhagawati, N. Bhattacharya, S. Borchetia and S. Das (2010). Herbivory Induced Transcriptome Changes in Tea. ‘Plant Genetics, Genomics and Biotechnology’ (June, 06-10, 2010), Russian Academy of Sciences, Novosibirsk, Russia.
11. B. Gohain, T. Bandyopadhyay, R. Bharalee, S. Gupta, P. Bhorali, S. K. Das, N. Agarwal, H.R. Singh, P. Ahmed, P. Bhagawati, N. Bhattacharya, C. Borah, S. Borchetia, and S. Das (2010). Deciphering the molecular trajectory in Darjeeling tea due to abiotic and biotic stress. ‘International Conference on Frontiers in Biological Sciences’ (Oct, 01-03, 2010), NIT, Rourkela.
12. B. Gohain, S. Borchetia, T. Bandyopadhyay, R. Bharalee, S. Gupta, P. Bhorali, S. K. Das, N. Agarwal, H.R. Singh, P. Ahmed, P. Bhagawati, N. Bhattacharya, C. Borah and S. Das (2010). Tea Quality: A Molecular Insight. ‘6^th Scandinavian Plant Physiologist Society PhD student conference’ (Sept, 02-05, 2010), University of Helsinki, Finland.
13. S. Borchetia, B. Gohain, C. Borah, N. Agarwal, P. Bhorali, T. Bandyopadhyay, S. Gupta, R. Bharalee, S.K. Das, H. R. Singh, P. Ahmed, P. Bhagawati, N. Bhattacharya and S. Das (2010). MeJa and salicyclic acid induced expression of jasmonate biosynthetic genes and associated defence genes in metabolites ofCamellia assamica ssp. Lasiocalyx. ‘International Conference on Frontiers in Biological Sciences’ (Oct, 01-03, 2010), NIT, Rourkela.

Research Experience

2. At The Energy and Resources Institute, Guwahati (Nov 2011 to Nov 2015)

• My responsibility mainly includes development and execution of project proposals to be submitted to different funding agencies and also to perform project based wet lab experiment for time bound deliverables.
• I was executing a DBT sponsored project as PI entitle "Molecular analysis of fruit development and ripening of Hippophae salicifolia of Sikkim Himalayas using High-throughput sequencing-based gene profiling" with a budget of 58 Lacs.
• I was also involved in identification and cloning of differentially expressed genes of Aquilaria malaccensis during Agarwood formation using AFLP techniques.
• I am also involved in identification and screening for potential biodiesel yielding microalgae from North East India.

3. At Tea Researh Association, Jorhat, India, (December 2006 to Nov 2011)

• As JRF/SRF: I was involved in identifying stress responsive genes of tea, in particular drought stress. I have constructed a numbers of suppression subtractive libraries (SSH) and identified a large nubers of differentially expressed genes in tea under different biotic and abiotic stresses.
• Cloning and Protein Expression: I have also isolated six drought induced full length gene (Acession no. JN561073- JN561077 and JN391508) through RACE PCR and cloned and expressed in E coli. The gene product was induced and protein was purified and visualize in PAGE gel.
•  Molecular biology techniques: I have gathered expertise in molecular biology techniques like DNA/RNA isolation, PCR, RT-PCR, Cloning and heterologous protein expression, cDNA library construction, SSH library construction, DNA sequencing using GA3130 xl Genetic Analyzer etc. Besides
• Bioinformatics: I have expertise in basic bioinformatics tools like BlastX, BlastP, Blastn, multiple sequence alignment, functional annotation of ESTs, primer designing in Primer 3.
• DNA sequencing Experience: I have sequenced several cDNA libraries and submitted 2300 ESTs to NCBI’s EST database (Accession no. HS393200 to HS395561).
• Worked as a senior research fellow from Jan, 2005 to July,2005 in Indian Council of Medical Research, Dibrugarh, where I studied the polymorphism of ACE gene related to hypertension in different ethnic groups of North East India.

Training

1. Completed 3 days hands on training on DNA sequencing and Fragment Analysis from Lab India, Gurgaon, Delhi, India.
2. Completed six months hands on training certificate course entitled “Post MSc certificate course in Plant Genetic Engineering” from Department of Biotechnology, Madurai Kamraj University (from Dr k Veluthambi's lab), Madurai. The course was focused mainly towards learning molecular biology techniques like DNA extraction, PCR, cloning, transformation, Western blotting, Southern hybridization, plasmid isolation, restriction  digestion, gel purification of DNA, GUS staining, basic tissue culture techniques, Agrobacterium mediated transformation, protein expression in E. coli, SDS-PAGE etc.
3. Completed three days Workshop on “Recent Trends in Bioinformatics”, NEHU, Shillong, Meghalaya.
4. Completed 5 days Training course on AFLP at TRA, Jorhat, Assam, India
5. Completed a three days training on In silico characterization of proteins from sequences and molecular modelling approaches  organised by CVSc. Khanapara from Nov 7-12, 2012
6. Completed four days workshop cum training programme on Next Generation Sequencing: Data analysis and annotation organized by Bioinformatics Centre, Indian Institute of Spice Research, Kozhikode from 17-20^th March, 2014.